IJMS, Free Full-Text
Por um escritor misterioso
Last updated 21 setembro 2024
A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The assembly employed a supramolecular oligonucleotide dendrimer as a 3D template that was obtained via the hybridization of siRNA strands and a single-stranded DNA oligonucleotide joined to three- or four-way branched junctions. A four-way branched oligonucleotide building block (a starlet) was designed for the assembly of the shell composed of three identical self-complementary DNA single strands and a single RNA strand for hybridization to the DNA oligonucleotides of the template. To prevent premature auto-hybridization of the self-complementary oligonucleotides in the starlet, a photolabile protecting group was introduced via the N3-substituted thymidine phosphoramidite. Cleavable linkers such as a disulfide linkage, RNase A sensitive triribonucleotides, and di- and trideoxynucleotides were incorporated into the starlet and template at specific points to guide the post-assembly disconnection of the shell from the template, and enzymatic disassembly of the template and the shell in biological media. At the same time, siRNA strands were modified with 2′-OMe ribonucleotides and phosphorothioate groups in certain positions to stabilize toward enzymatic digestion. We report herein a solid-phase synthesis of branched oligodeoxy and oligoribonucleotide building blocks for the DNA/RNA dendritic template and the branched DNA starlet for a template-assisted construction of a C24 fullerene-like DNA shell after initial molecular modeling, followed by the assembly of the shell around the DNA-coated RNA dendritic template, and visualization of the resulting nanostructure by transmission electron microscopy.
IJMS, Free Full-Text
IJMS, Free Full-Text
IJMS, Free Full-Text
IJMS, Free Full-Text
IJMS Free Full-Text Immunoglobulin Abnormalities In Gaucher, 48% OFF
Ijms Free Full Text Non Invasive Detection Of Extracellular Matrix
IJMS, Free Full-Text
Ijms Free Full Text The Macrophages Microbiota Interplay In
Ijms Free Full Text Approaching Sex Differences In Cardiovascular
IJMS, Free Full-Text
Plant Abiotic Stress
IJMS, Free Full-Text
Pat Testing Labels Template Unique Ijms Free Full Text Genome
Recomendado para você
-
O QUE É CGC? Brechó, Dicas de look, Brechós21 setembro 2024
-
Coliscan® MF Colony Color Guide21 setembro 2024
-
11 Weeks of TWD – Season 4 by Christine Norrie Premium Gallery Wrapped Canvas21 setembro 2024
-
Do Souto Cavaquinho, Waldir, 1976 (SOLD) – Choro Shop21 setembro 2024
-
Marcio Ruiz - Franklin, Tennessee, United States21 setembro 2024
-
Meet Rachel Williamson, MS, CGC - Genetic Center - Akron21 setembro 2024
-
The Me You Love in the Dark #1 | 1:25 Incentive Ratio Variant | CGC SS 9.421 setembro 2024
-
jorge degas & marcelo salazar – muxima21 setembro 2024
-
Vipermagi from norm Baal mf runs! :D what an exciting way to start21 setembro 2024
-
Females — PRIMA Dobermans21 setembro 2024
você pode gostar
-
MangaOwl is a popular online platform that offers a vast library of manga titles for free. MangaOwl App, MangaOwl net, MangaOw…21 setembro 2024
-
Milan x PSG pela Champions League 2023/24: onde assistir ao vivo21 setembro 2024
-
Jogo da Copinha foi a julgamento e Araucária corre o risco de ser eliminado - O Popular do Paraná21 setembro 2024
-
Electronic Arts: Battlefield 4 - Sony PlayStation 3 Video Game21 setembro 2024
-
Culture & Trends - The year in Gaming21 setembro 2024
-
Equipamentos Fabrica De Bolo Caseiro21 setembro 2024
-
Minecraft: Story Mode coming to Netflix, Telltale plans Stranger21 setembro 2024
-
Mulher-Hulk' enfrenta problemas criativos na pós-produção, diz jornalista21 setembro 2024
-
Etiqueta Escolar Tilibra Gato Galáctico - Art Pel21 setembro 2024
-
Dog Ate Mucinex21 setembro 2024